unmethylated In A Sentence
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- BstUI ), which will only cleave sites that were originally methylated ( CGCG ), while leaving sites that were originally unmethylated ( TGTG ).
- Therefore, PCR products resulting from a template that was originally unmethylated will have a lower melting point than those derived from a methylated template.
- If conversion is incomplete, the subsequent analysis will incorrectly interpret the unconverted unmethylated cytosines as methylated cytosines, resulting in false positive results for methylation.
- It relies upon the selective deamination of unmethylated cytosine to uracil after treatment with sodium bisulphite, usually followed by PCR amplification of the chosen target region.
- CTCF binds to the unmethylated maternal ICR region but not the methylated paternal ICR region.
- This detail gives the opportunity to researchers to differentiate a methylated allele from the unmethylated one.
- The H19 locus remains unmethylated on the maternal allele and allows for binding of CTCF to the DMR, inhibiting IGF2 as it cannot access the common enhancers.
- This is because adenine introduced into the new DNA strand is unmethylated.
- However, " HpaII " requires that a C within that site be methylated, whereas " MspI " cleaves only DNA unmethylated at that site.
- The majority of CpG islands are constitutively unmethylated and enriched for permissive chromatin modification such as H3K4 methylation.
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